Free Demo Syringe Column!!!
Just pay shipping and write review after testing.
What is a Syringe Column?
Syringe columns are manual, syringe-based affinity chromatography devices designed for cost-effective protein extraction. The user loads, washes, and elutes target proteins by aspirating and dispensing solutions through the embedded affinity resin. This method allows for efficient equilibrium binding and is suitable for applications such as antibody purification. The columns are reusable for up to 10 extractions and eliminate the need for specialized equipment, providing a simpler alternative to techniques like magnetic bead, gravity, or spin column chromatography. Why are these cost effective? Simply cap the column when the purification is complete and it is ready for the next purification. Syringe columns can be used for up to 10 purifications
Why Use Syringe Column
1. Intuitive and Easy to Use.
2. Resin/Columns can be regenerated and resued up to at least 10 times.
3. Columns can be capped and stored indefinitely (the resin never dries out).
4. Manually operated - no equipment needed.​
5. Most cost effective protein purification technology available.
6. Obtain up to 1mg of recombinatn protein or mAb.
7. No Clogging.
8. No Gravity, no Magnets, no Centrifuge.
9. Back and forth flow requires all solvents to interaction to completion.​
10. Purified proteins compatible with downstream assay activies.
Recombinant Protein Purification Results
High efficient binding and recovery of His-GFP using Syringe ColumnsThe flow through of the sample was monitored using the NanoDrop after 8, 12 and 16 aspirate and dispense cycles. The majority of the His-GFP was recovered in elution 1 (Table 1). Elution 2 recovered about 1.2 mg of protein for a total of almost 7 mg of His-GFP.
Syringe column instruction
1. Equilibrate Syringe Column:
Transfer 1 mL of 1X TBS to a 1.5 mL microfuge tube. Uncap syringe column and attached the luer lock needle. Aspirate 1 mL of air into the syringe column. Submerge the needle into the tube and aspirate 1 mL of TBS at a rate of 1 mL in 3 seconds. Dispense 1 mL. Repeat for a fresh aliquot of 1 mL 1X TBS is desired. Blowout 1 mL of air to remove the interstitial fluid
2. Capture Sample:
Aspirate 1 mL of air into the syringe column. Lower the needle into the 1 mL sample tube. Perform 16-24 cycles of capture as per Step (1). After the final capture dispense, blowout 1 mL of air.
3.Wash1:
Aspirate 1 mL of air into the syringe column. Lower the needle into 1 mL of 1X TBS. Perform 2 cycles of wash as per Step (1). After the final wash dispense, blow out 1 mL of air.
4. Wash2:
Aspirate 1 mL of air into the syringe column. Lower the needle into 1 mL of 10 mM NaCl. Perform 2 cycles of wash as per Step (1). After the final wash dispense, blow out 1 mL of air.
5. Elution1:
Aspirate 1 mL of air into the syringe column. Lower the needle into 1 mL of Elution Buffer (Thermo Scientific, 21030). Perform 8 cycles of Elution as per Step (1). After the final elution dispense, blow out 1 mL of air.​
6. Elution2:
Aspirate 1 mL of air into the syringe column. Lower the needle into a fresh aliquot of 1 mL of Elution Buffer to perform the second elution. Perform 8 cycles of elution as per Step (1). After the final elution dispense, blow out 1 mL of air.
WELCOME
Welcome to DTG Biotech, a leading Biotechnology Manufacturer based in San Jose, CA. With over 40 years of experience, we pride ourselves on being innovators of the highest standard, using cutting-edge technologies to lead our industry forward. Through our dedicated and meticulous work, our company has earned a reputation as a top provider of syringe columns for purification, depletion, and assays.